聽聽聽
聽聽聽聽聽ANTIBIOTICS—MICROBIAL聽ASSAYS聽(抗生素的微生物检测)
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIA聽AND聽DILUENTS（培养基和稀释剂）
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽Media
聽聽聽The聽media聽required聽for聽the聽preparation聽of聽test聽organism聽inocula聽are聽made聽
from聽the聽ingredients聽listed聽herein.聽Minor聽modifications聽of聽the聽individual聽
ingredients,聽or聽reconstituted聽dehydrated聽media,聽may聽be聽substituted,聽provided聽
the聽resulting聽media聽possess聽equal聽or聽better聽growth-promoting聽properties聽and聽
give聽a聽similar聽standard聽curve聽response.
聽聽聽Dissolve聽the聽ingredients聽in聽water聽to聽make聽1聽L,聽and聽adjust聽the聽solutions聽
with聽either聽1聽N聽sodium聽hydroxide聽or聽1聽N聽hydrochloric聽acid聽as聽required,聽so聽that聽
after聽steam聽sterilization聽the聽pH聽is聽as聽specified.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽1

Peptone 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽6.0聽g
Pancreatic聽Digest聽of聽Casein 4.0聽g
Yeast聽Extract 聽聽聽聽聽聽聽聽聽3.0聽g
Beef聽Extract 聽聽聽聽聽聽聽聽聽1.5聽g
Dextrose 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1.0聽g
Agar 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽15.0聽g
Water 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1000聽mL
聽聽pH聽after聽sterilization:聽6.6聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽2

Peptone 聽聽聽聽聽聽聽聽聽6.0聽g
Yeast聽Extract 3.0聽g
Beef聽Extract 1.5聽g
Agar 聽聽聽聽聽聽聽聽聽15.0聽g
Water 聽聽聽聽聽聽聽聽聽1000聽mL
pH聽after聽sterilization:聽6.6聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽3

Peptone 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽5.0聽g
Yeast聽Extract 聽聽聽聽聽聽聽聽聽1.5聽g
Beef聽Extract 聽聽聽聽聽聽聽聽聽1.5聽g
Sodium聽Chloride 聽聽聽聽聽聽聽聽聽3.5聽g
Dextrose 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1.0聽g
Dibasic聽Potassium聽Phosphate 3.68聽g
Monobasic聽Potassium聽Phosphate 1.32聽g
Water 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1000聽mL
pH聽after聽sterilization:聽7.0聽±聽0.05.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽4
聽聽聽Same聽as聽Medium聽2,聽except聽for聽the聽additional聽ingredient聽1.0聽g聽of聽Dextrose.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽5
聽聽聽Same聽as聽Medium聽2,聽except聽that聽the聽final聽pH聽after聽sterilization聽is聽7.9聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽8
聽聽聽Same聽as聽Medium聽2,聽except聽that聽the聽final聽pH聽after聽sterilization聽is聽5.9聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽9

Pancreatic聽Digest聽of聽Casein 17.0聽g
Papaic聽Digest聽of聽Soybean 3.0聽g
Sodium聽Chloride 聽聽聽聽聽聽聽聽聽5.0聽g
Dibasic聽Potassium聽Phosphate 2.5聽g
Dextrose 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽2.5聽g
Agar 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽20.0聽g
Water 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1000聽mL
聽聽聽pH聽after聽sterilization:聽7.2聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽10
聽聽聽Same聽as聽Medium聽9,聽except聽to聽use聽12.0聽g聽of聽Agar聽instead聽of聽20.0聽g,聽and聽to聽add聽10聽mL聽of聽Polysorbate聽80聽after聽boiling聽the聽medium聽to聽dissolve聽the聽agar.
聽聽聽pH聽after聽sterilization:聽7.2聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽11
聽聽聽Same聽as聽Medium聽1,聽except聽that聽the聽final聽pH聽after聽sterilization聽is聽8.3聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽13

Dextrose 20.0聽g
Peptone 10.0聽g
Water 1000聽mL
聽聽聽pH聽after聽sterilization:聽5.6聽±聽0.1.
聽
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽19

Peptone 聽聽聽聽聽聽聽聽聽9.4聽g
Yeast聽Extract 4.7聽g
Beef聽Extract 2.4聽g
Sodium聽Chloride 10.0聽g
Dextrose 聽聽聽聽聽聽聽聽聽10.0聽g
Agar 聽聽聽聽聽聽聽聽聽23.5聽g
Water 聽聽聽聽聽聽聽聽聽1000聽mL
聽聽聽pH聽after聽sterilization:聽6.1聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽32
聽聽聽Same聽as聽Medium聽1,聽except聽for聽the聽additional聽ingredient聽0.3聽g聽of聽Manganese聽Sulfate.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽34

Glycerol 聽聽聽聽聽聽聽聽聽10.0聽g
Peptone 聽聽聽聽聽聽聽聽聽10.0聽g
Beef聽Extract 10.0聽g
Sodium聽Chloride 3.0聽g
Water 聽聽聽聽聽聽聽聽聽1000聽mL
聽聽聽聽pH聽after聽sterilization:聽聽聽聽7.0聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽35
聽聽Same聽as聽Medium聽34,聽except聽for聽the聽additional聽ingredient聽17.0聽g聽of聽Agar.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽36

Pancreatic聽Digest聽of聽Casein 15.0聽g
Papaic聽Digest聽of聽Soybean 5.0聽g
Sodium聽Chloride 聽聽聽聽聽聽聽聽聽5.0聽g
Agar 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽15.0聽g
Water 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1000聽mL
pH聽after聽sterilization:聽聽聽聽聽聽聽聽聽聽聽聽聽聽7.3聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽39
聽聽聽Same聽as聽Medium聽3,聽except聽that聽the聽final聽pH聽after聽sterilization聽is聽7.9聽±聽0.1.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽40

Yeast聽Extract 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽20.0聽g
Polypeptone 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽5.0聽g
Dextrose 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽10.0聽g
Monobasic聽Potassium聽Phosphate 2.0聽g
Polysorbate聽80 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽0.1聽g
Agar 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽10.0聽g
Water 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1000聽mL
聽聽聽pH聽after聽sterilization:聽6.7聽±聽0.2.
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽MEDIUM聽41

Pancreatic聽Digest聽of聽Casein 9.0聽g
Dextrose 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽20.0聽g
Yeast聽Extract 聽聽聽聽聽聽聽聽聽5.0聽g
Sodium聽Citrate 聽聽聽聽聽聽聽聽聽10.0聽g
Monobasic聽Potassium聽Phosphate 1.0聽g
Dibasic聽Potassium聽Phosphate 1.0聽g
Water 聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽1000聽mL
聽聽聽pH聽after聽sterilization:聽6.8聽±聽0.1.
聽聽聽聽聽聽Phosphate聽Buffers聽and聽Other聽Solutions聽（磷酸盐缓冲液和其他的解决办法）
聽聽Prepare聽as聽follows,聽or聽by聽other聽suitable聽means,聽the聽potassium聽phosphate聽
buffers聽required聽for聽the聽antibiotic聽under聽assay.聽The聽buffers聽are聽sterilized聽
after聽preparation,聽and聽the聽pH聽specified聽in聽each聽case聽is聽the聽pH聽after聽
sterilization.

聽聽聽BUFFER聽NO.聽1,聽1聽PERCENT,聽pH聽6.0—聽Dissolve聽2.0聽g聽of聽dibasic聽potassium聽
phosphate聽and聽8.0聽g聽of聽monobasic聽potassium聽phosphate聽in聽1000聽mL聽of聽water.聽
Adjust聽with聽18聽N聽phosphoric聽acid聽or聽10聽N聽potassium聽hydroxide聽to聽6.0聽±聽0.05.
BUFFER聽NO.聽3,聽0.1聽M,聽pH聽8.0—聽Dissolve聽16.73聽g聽of聽dibasic聽potassium聽
phosphate聽and聽0.523聽g聽of聽monobasic聽potassium聽phosphate聽in聽1000聽mL聽of聽water.聽
Adjust聽the聽pH聽with聽18聽N聽phosphoric聽acid聽or聽10聽N聽potassium聽hydroxide聽to聽8.0聽±聽0.1.
聽
聽聽BUFFER聽NO.聽4,聽0.1聽M,聽pH聽4.5—聽Dissolve聽13.61聽g聽of聽monobasic聽potassium聽
phosphate聽in聽1000聽mL聽of聽water.聽Adjust聽the聽pH聽with聽18聽N聽phosphoric聽acid聽or聽10聽N聽
potassium聽hydroxide聽to聽4.5聽±聽0.05.
聽
聽聽BUFFER聽NO.聽6,聽10聽PERCENT,聽pH聽6.0—聽Dissolve聽20.0聽g聽of聽dibasic聽potassium聽
phosphate聽and聽80.0聽g聽of聽monobasic聽potassium聽phosphate聽in聽1000聽mL聽of聽water.聽
Adjust聽the聽pH聽with聽18聽N聽phosphoric聽acid聽or聽10聽N聽potassium聽hydroxide聽to聽6.0聽±聽0.05.

聽聽BUFFER聽NO.聽10,聽0.2聽M,聽pH聽10.5—聽Dissolve聽35.0聽g聽of聽dibasic聽potassium聽
phosphate聽in聽1000聽mL聽of聽water,聽and聽add聽2聽mL聽of聽10聽N聽potassium聽hydroxide.聽
Adjust聽the聽pH聽with聽18聽N聽phosphoric聽acid聽or聽10聽N聽potassium聽hydroxide聽to聽10.5聽±聽0.1.

聽聽BUFFER聽NO.聽16,聽0.1聽M,聽pH聽7.0—聽Dissolve聽13.6聽g聽of聽dibasic聽potassium聽phosphate聽and聽4.0聽g聽of聽monobasic聽potassium聽phosphate聽in聽1000聽mL聽of聽water.聽
Adjust聽with聽18聽N聽phosphoric聽acid聽or聽10聽N聽potassium聽hydroxide聽to聽a聽pH聽of聽7.0聽±聽
0.2.

聽聽OTHER聽SOLUTIONS—聽Use聽the聽substances聽specified聽under聽Reagents,聽Indicators,聽
and聽Solutions.聽For聽water,聽use聽Purified聽Water.聽For聽saline,聽use聽Sodium聽Chloride聽
Injection.聽Dilute聽formaldehyde聽is聽Formaldehyde聽Solution聽diluted聽with聽water聽1:3.

聽

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